Pyrosequencing - Wikipedia
网页The pyrosequencing method is based on detecting the activity of DNA polymerase (a DNA synthesizing enzyme) with another chemoluminescent enzyme. Essentially, the method allows sequencing a single strand of DNA by synthesizing the complementary strand along it, one base pair at a time, and detecting which base was actually added at each step.
Pyrosequencing Technology and Platform Overview - QIAGEN
网页Pyrosequencing is a sequence-based detection technology that enables rapid and accurate quantification of sequence variation. Streamlined protocols, analysis flexibility, and elegant output make Pyrosequencing technology a highly adaptable tool for exploratory and testing work in a broad range of disciplines.
焦磷酸测序 - 百度百科
网页焦磷酸测序技术(pyrosequencing)是一种新型的酶联级联测序技术，焦磷酸测序法适于对已知的短序列的测序分析，其可重复性和精确性能与SangerDNA测序法相媲美，而速度却大大的提高。
Pyrosequencing: Principle, Steps, Reactions, Types, Uses
网页2024年8月4日 · Pyrosequencing is a sequencing method that determines the sequence of nucleotides by detecting the release of pyrophosphate (PP i) during nucleotide incorporation and monitors DNA synthesis in real-time. Pyrosequencing relies on light detection which occurs due to a chain reaction initiated by the release of pyrophosphate.
焦磷酸测序（Pyrosequencing）原理与应用 - MedSci
网页焦磷酸测序（Pyrosequencing）技术是由4种酶（DNA 聚合酶(DNA polymerase)、ATP硫酸化酶(ATP sulfurytase)、荧光素酶(luciferase)和三磷酸腺苷双磷酸酶(Apyrase)）催化的同一反应体系中的酶级联化学发光反应。
Pyrosequencing - an overview | ScienceDirect Topics
网页Pyrosequencing is a nongel-based DNA sequencing technique that detects inorganic pyrophosphate released during DNA synthesis, allowing for faster, cheaper, and semiquantitative analysis of nucleotide incorporation compared to traditional methods like Sanger sequencing.
What is Pyrosequencing? - News-Medical.net
网页2018年10月31日 · Pyrosequencing is a method of DNA sequencing that detects light emitted from nucleotide bases. By comparing the relative amount of light emitted, the sequence is uncovered.
5.12: Pyrosequencing - Biology LibreTexts
网页2022年5月14日 · Its method is called pyrosequencing or sequencing by synthesis. It works like this. The DNA to be sequenced is broken up into fragments of ~100 base pairs and denatured to form single-stranded DNA (ssDNA).
Pyrosequencing: powerful and quantitative sequencing technology
网页2023年7月15日 · Pyrosequencing is a sequencing-by-synthesis method for DNA analysis that has emerged as a platform not only for de novo sequencing applications, but also for quantitative analysis of genomic methylation, single-nucleotide polymorphisms, and allele quantification.
Pyrosequencing » Genetic Resources Core Facility, Johns Hopkins ...
网页Pyrosequencing is a method of sequencing that relies on light emitted in an enzymatic reaction set in motion by the release of pyrophosphate when a base is added during the sequencing process. The results are quantitative, making the method ideal for sequencing samples of known sequence that may have mutations in …
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