Rapid qualitative evaluation of DNA transcription factor NF-κB by ...
网页We have developed a separation technique for DNA-protein complex based on electrophoretic mobility shift assay (EMSA) by microchip electrophoresis, which we call microchip electrophoretic mobility shift assay (μEMSA). To evaluate the μEMSA, we employed recombinant human nuclear factor-κB (rhNF …
Effects of Different Ligands in the Notch Signaling Pathway on the ...
网页2020年8月6日 · RhNF-κB is an activator of the Notch signaling pathways; it cannot activate the Notch receptor, but it can promote the expression of Jagged1 and activate the Notch pathway indirectly .
An Ethylene-inhibited NF-YC Transcription Factor RhNF-YC9 …
网页2020年11月1日 · Ethylene functions as a negative regulator involved in the regulation of the petal expansion process. In this study, we isolated a NF-YC transcription factor gene, RhNF-YC9, from rose petals. RhNF-YC9 expression was induced at the early stages of flower opening but was inhibited by ethylene treatment.
Effects of Different Ligands in the Notch Signaling Pathway on the ...
网页2020年8月6日 · In response to rhNF-κB, the cell number and proliferation were reduced, and mRNA and protein levels of Jagged1 and Notch1 were up-regulated, while Dlk1, and SP-C were downregulated. In the Dlk1 group, SP-C, and AQP5 expression patterns suggested that the cells were still transdifferentiating by 96 h, while in the rhNF-κB group, most cells had ...
【求助】Notch激活剂-丁香实验 - biomart.cn
网页2015年8月20日 · 现在有研究者使用Recombinant Rat Jagged 1 Fc Chimera，作为Notch的激活剂，不知在选择上有何不同？. yuncen. 忘记从哪看到 EDTA可以激活notch通路，当然非特异. 本文由丁香园论坛提供，想了解更多有用的、有意思的前沿资讯以及酷炫的实验方法的你，都可以成为师兄的好 ...
基于Notch信号通路探讨β-榄香烯逆转肺腺癌耐药机制的研究
网页A549/DDP细胞在β-榄香烯处理的基础上，联合1 μg·mL-1 Notch信号通路激活剂rhNF-κB处理，比较两组的IC 50 ，Western blotting检测P-gp以及Notch1、Hes1、Hey2的表达。 结果 当β-榄香烯浓度低于20 μg·mL -1 时，其对A549/DDP细胞的抑制率小于10%，无细胞毒性。
Wnt and Notch signaling pathway involved in wound healing …
网页2015年6月16日 · Although rhNF-κB is not a Notch agonist, it can trigger the Notch signaling pathway by directly inducing the expression of Jagged1. DAPT, a γ-secretase inhibitor, inhibits Notch signaling by blocking the cleavage of NICD necessary for activation of transcription of downstream target genes.
Trends in Management of Radial Head and Olecranon Fractures
网页2017年3月31日 · The purpose of this study is to examine the national trends for management of two common elbow fractures-1) radial head and neck fractures (RHNF); 2) olecranon fractures – using the Humana claims database between 2007 and 2014.
Dlk1、Jagged1基因在大鼠肺泡Ⅱ型上皮细胞增殖及转分化中的作 …
网页重组人核录因子－κB(rhNF－κB)是一种Notch信号通路的激活剂，其本身不能激活Notch受体，但可以促进Jagged1的表达而激活Notch通路 。本实验通过在原代细胞培养体系中促进不同配体的表达，研究其对AECⅡ的转分化及增殖的作用。
NS5ATP9 gene regulated by NF-κB signal pathway - ScienceDirect
网页2008年11月1日 · We found that the nuclear protein rhNF-κB (p50) could bind to the NS5ATP9 promoter and the binding region contained within a 156 bp (nucleotides −5 to −161 bp) immediately upstream of the transcription initiation site. Our results suggest that NF-κB could participate in the regulation of NS5ATP9 gene expression in carcinogenesis.