Establishment and application of a VP3 antigenic domain-based …
网页2023年11月23日 · Reported ELISA methods for detecting antibody against GPV infection mainly include ELISA that utilize VP3 full-length protein as the coating antigen (Zhang et al., 2010), ELISA that use VP3 antigen site VP3ep4–6 recombinant protein as the coating antigen (Tarasiuk et al., 2019), and ELISA that employ …
Development and evaluation of a VP3-ELISA for the detection of …
网页The VP3 fusion protein expressed in E. coli was detected by goose and Muscovy duck anti-parvovirus polyclonal sera. In addition, an ELISA (VP3-ELISA) using the VP3 protein as the coating antigen for the detection of antibodies to GPV in geese and antibodies to Muscovy duck parvovirus (MDPV) in Muscovy ducks was developed.
Research Note: Development of an ELISA to distinguish between …
网页2020年1月25日 · In this study, an NS1-ELISA test and a VP3-ELISA test were developed to diagnose GPV infection and to monitor serum antibody titers against GPV. It distinguishes between the GPV infection and vaccine immunization antibodies and lays the foundation for the diagnosis of GPV disease and the serological investigation.
Establishment and application of a VP3 antigenic domain-based …
网页2023年11月23日 · In this study, bioinformatics software was used to analyze and predict the dominant antigenic regions of the main protective antigen VP3 of GPV. Three segments of bovine serum albumin (BSA) vector-coupled peptides …
The development of an indirect ELISA for the detection of goose ...
网页2019年8月1日 · In this paper, two immunodominant fragments of the VP3 protein of GPV (VP3ep6 and VP3ep4–6) were expressed and purified. An indirect ELISA was developed and compared with VN and another ELISA test. Finally, one of the purified proteins, VP3ep4–6, was used as the coating antigen in a new ELISA assay.
Development and evaluation of a VP3-ELISA for the detection of …
网页The dynamic antibody detection results of gosling vaccines and the testing of 120 clinical immune goose serum samples collectively demonstrate that BSA-VP3-1 peptide ELISA can be used to detect antibody against GPV in the immunized goose population and has higher sensitivity than traditional agar gel precipitation methods.
[PDF] Establishment and application of a VP3 ... - Semantic Scholar
网页2023年11月23日 · In this study, bioinformatics software was used to analyze and predict the dominant antigenic regions of the main protective antigen VP3 of GPV. Three segments of bovine serum albumin (BSA) vector-coupled peptides …
<br>基于VP3抗原域的鹅瘟病毒感染抗体检测肽ELISA ... - X-MOL
网页本研究利用生物信息学软件对GPV主要保护性抗原VP3的优势抗原区域进行分析和预测。. 合成了三段牛血清白蛋白 (BSA) 载体偶联肽作为 ELISA 包被抗原。. 实验结果表明，VP3-1（358-392aa）肽具有最好的反应性和特异性。. 利用BSA-VP3-1肽建立了抗GPV感染抗体 …
Development of a Novel Assay Based on Plant-Produced ... - PubMed
网页2021年12月7日 · In this study, an engineered version of the VP3 protein of IBDV (His-VP3) was produced in plants, successfully purified from Nicotiana benthamiana leaves, and used to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of anti-VP3 antibodies. The His-VP3 ELISA was validated with a panel of 180 reference sera and demonstrated ...
Assessing the antigenicity of different VP3 regions of infectious ...
网页2021年7月30日 · We tested the suitability of recombinant VP3 (rVP3) as a diagnostic reagent in an enzyme-linked immunosorbent assay (ELISA). Compared with a commercial test, rVP3 ELISA showed high sensitivity and specificity as …