Cloning promises to transform the efficiency of transgenesis from a dismal 1–5% using pronuclear injection, the current method of choice, to 100%. It will also significantly reduce the time ...

A nearly extinct whale helped him build a coalition that has played a part in slowing the rollout of wind turbines up and ...

The first transgenic animals were produced more than 50 years ago (Jaenisch &Mintz, 1974;Gordon et al., 1980) and now transgenic technology is successfully and broadly used in different fields of ...

F1-hybrid mouse strain: The C57BL/6 x DBA/2 F1 hybrid strain has been used in this core for standard transgene microinjections. Under the terms of the agreement, we will produce at least 30 offspring ...

For pronuclear microinjections (projects with dsDNA repair templates) the guide RNA(s) and Cas9 protein will be complexed into RNPs and the microinjection mix will be prepared with the repair template ...

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CRISPR/Cas-initiated HR in mouse embryos will be used to generate founder animals harboring inserted sequence targeted to the ROSA26 locus. The targeting will be done in embryos using microinjection ...

At the center of this progression is a suite of cell cycle regulatory proteins and molecular machines that drive and integrate processes such as chromosome segregation, fertilization, and pronuclear ...

Sky News host Chris Kenny says Facebook has tried to “play down” the issue of censoring posts promoting Nuclear for Australia. “Facebook is under fire for censoring posts promoting Nuclear ...

Lymphocyte activation gene (Lag)-3 is an inhibitory co-receptor and target of immune checkpoint inhibitor (ICI) therapy for cancer. The dynamic behavior of Lag-3 was analyzed at the immune synapse ...

During organogenesis, precise control of spindle orientation balances proliferation and differentiation. In the developing murine epidermis, planar and perpendicular divisions yield symmetric and ...